Wei Liu

I am a researcher in cryobiology and bioengineering, with interests in cell cryopreservation, vitrification, biobanking, and low-temperature injury mechanisms. My work focuses on developing safer and more effective cryopreservation strategies for clinically relevant cells and tissues, including human T lymphocytes, mesenchymal stem cells, and related biomedical samples.

Email / Google Scholar

Recent Projects

Cryoinjuries of Cells During Freezing

Simulation and visualization of the response of human T cells under different cooling rates.

Dynamic Simulation of Ice Crystal Growth

Visualization of the growth process of a single ice crystal under low-temperature conditions.

Talks

08/2018, Preliminary study on controlled ice nucleation of human T lymphocytes cryopreservation, The 14th National Refrigerator (Cabinet), Air Conditioner and Compressor Academic Annual Conference & the 11th National Cryo-Biomedical and Instrument Academic Annual Conference, Qingdao, China.

05/2019, Application of Apoptotic Inhibitors on the Cryopreservation of Cultured Human T Cells, ISBER 2019 Annual Meeting & Exhibits, Shanghai, China.

09/2019, Impacts of Trehalose and L-proline on the Thermodynamic Nonequilibrium Phase Change and Thermal Properties of Normal Saline, 2019 International Symposium on Cryobiology and Living Biobanking & Forum on Innovation for College Students, Changsha, China.

12/2020, Challenges and strategies in the cryopreservation of cells, The 3rd China Drug Safety Technology Conference & the 4th Biosafety Forum, Beijing, China.

Research

I am interested in developing freezing protocols for different types of biosamples, including human T lymphocytes, umbilical cord mesenchymal stem cells, and umbilical cord tissues.

Research Papers

Review of the Mechanism of Intracellular Ice Formation
Wei Liu, Mingwei Guo, Zhiyu Guo, Baolin Liu
Journal of Refrigeration (in Chinese) 2018/06/1 | paper
Impacts of trehalose and l-proline on the thermodynamic nonequilibrium phase change and thermal properties of normal saline
Wei Liu, Zhiyong Huang, Xiaowen He, Pei Jiang, Xiaoyue Huo, Zekang Lu, Baolin Liu
Cryobiology 2020/10/1 | paper
Investigating solution effects injury of human T lymphocytes and its prevention during interrupted slow cooling
Wei Liu, Zhiyong Huang, Baolin Liu, Xiaowen He, Suxia Xue, Xiaojuan Yan, Ganesh K Jaganathan
Cryobiology 2021/4/1 | paper
Optimization of UC-MSCs cold-chain storage by minimizing temperature fluctuations using an automatic cryopreservation system
Yanhong Xu, Leo Li-Ying Chan, Siye Chen, Bi Ying, Ting Zhang, Wei Liu, Hao Guo, Jianxin Wang, Zhifeng Xu, Xuebing Zhang, Xiaowen He
Cryobiology 2021/4/1 | paper
Cryopreservation of human T lymphocytes under fast cooling with controlled ice nucleation in cryoprotective solutions of low toxicity
Zhiyong Huang, Wei Liu, Baolin Liu, Xiaowen He, Hao Guo, Suxia Xue, Xiaojuan Yan, Ganesh K Jaganatha
Cryobiology 2021/12/1 | paper
Slow Cooling and Controlled Ice Nucleation Enabling the Cryopreservation of Human T Lymphocytes with Low-Concentration Extracellular Trehalose
Zhiyong Huang, Wei Liu, Tianhua Ma, Honglian Zhao, Xiaowen He, Baolin Liu
Biopreservation and Biobanking 2022/8/1 | paper

Education

2011.09 - 2015.07: Bachelor of Biological Science, China Agricultural University.

2016.09 - 2022.06: Doctor of Biomedical Engineering, University of Shanghai for Science and Technology.

Awards

2017, National Bronze Winner, The Third China College Students "Internet +" Innovation and Entrepreneurship Competition.

2019, Outstanding Prize of Presentation, 2019 International Symposium on Cryobiology and Living Biobanking & Forum on Innovation for College Students.

Low-Temperature Biology Tools

Measure osmotic permeability

Find the best cooling rate for your cell